Researched Technology
Determination of Novel Plant Growth Promoting Bacteria Stenotrophomonas maltophilia Isolated from Rhizospheric Soils of South Gujarat Forest, India
Forest regions have good weather conditions for agricultural practices. Crop growth and productivity are affected by the presence of novel bioorganisms in these areas. The present study sought to identify the location of the forest rhizosphere soil of some field crops. Screening procedures were used to assess their growth capabilities and evaluate their potential as PGPB. Various features of PGPB, like phosphate and potassium solubilization, nitrogen fixation, indole-3-acetic acid (IAA) production, ammonia (NH3) synthesis, siderophore generation, and hydrogen cyanide (HCN) synthesis, were analyzed morphologically, biochemically, and physiologically during the screening process. One isolate out of the nine only produced good results for every attribute studied that promoted plant development. By sequencing their 16S rRNA, the promising isolates were determined to be Stenotrophomonas maltophilia. To assess the efficacy of PGPB and its liquid and powder formulation as a biofertilizer, a field trial had been conducted. In comparison to the uninoculated control, all tested inoculants considerably enhanced the yield
components of several crops, the amount of N and P that plants could absorb from the soil, the activity of some soil enzymes, and the overall number of bacteria. The application of Stenotrophomonas maltophilia as a PGPR demonstrated significant promise in addressing the obstacles associated with sustainable agriculture in a variety of environmental circumstances.
View Research PDFIsolation, Identification, and Characterization of Plant Growth Promoting Rhizobacteria from Sugarcane (Saccharum Officinarum) Rhizosphere
The use of biofertilizers and biopesticides is the foundation of modern sustainable agricultural techniques. The rhizosphere of sugarcane (Saccharum officinarum) may provide rhizobacteria the ability to fertilize and repel pests. This research focused at the plant growth promotion (PGP) abilities of bacteria found in the rhizosphere of sugarcane. For example, how they solubilize phosphate, produce IAA, produce nitrogen, and help seeds sprout. Soil samples were taken from different types of sugarcane rhizosphere in the Ankleshwar area to obtain isolates. For further study and molecular identification using the 16S rRNA gene sequence, one strain of bacteria was picked because it could solubilize phosphate and produce IAA. This confirms the isolate's identity as Klebsiella pneumoniae. The isolate had a phosphate solubilization index of 2.11, and the PKVK broth gave off 91.34±2 μg/ml of phosphate, which was calculated. After 72 hours of incubation at room temperature, 61.54±2 μg/ml of IAA without tryptophan was generated. The test on seed germination showed that treating plants with Glycine max, Solanum melongena,
Solanum lycopersicum, Capsicum annuum, and Oryza sativa bacteria increased plant height, dry weight, and fresh weight more than the control group. Solanum lycopersicum exhibited the highest rate of germination (100%). The infected agricultural seeds' better seedling characteristics suggested that this isolate may be used as a biofertilizer formulation for environmentally friendly production.
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